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flow cytometry analysis
Flow Cytometry Analysis, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1579 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+analysis/CytoFLEX+LX+Flow+Cytometer/pmc13623122-436-2-11
Average 96 stars, based on 1579 article reviews
Flow Cytometry Analysis, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1579 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+analysis/CytoFLEX+LX+Flow+Cytometer/pmc13623122-436-2-11
Average 96 stars, based on 1579 article reviews
flow cytometry analysis - by Bioz Stars,
2026-10
96/100 stars
Images
1) Product Images from "Elevated Plasmodium falciparum sexual conversion in HbAC and HbAS red blood cells in naturally infected malaria patients"
Article Title: Elevated Plasmodium falciparum sexual conversion in HbAC and HbAS red blood cells in naturally infected malaria patients
Journal: PLOS Pathogens
doi: 10.1371/journal.ppat.1014542
Figure Legend Snippet: (A) Experimental design of in vitro SC assays ( iv SCAs) using the NF54 -gexp02-Tom P. falciparum line. Tightly synchronized schizonts (Generation -1) were allowed to reinvade red blood cells (RBCs) of different HBB genotypes, and ring stages (0-5 hpi) were cultured under non-inducing (+Choline) and inducing (-Choline) conditions. Parasite development was assessed by flow cytometry at 35-40 hpi (Generation 0) and 25-30 hpi/35-40 hpi (Generation 1). SCRs were measured by flow cytometry at 25-30 hpi and 35-40 hpi (Generation 1). SCRs by HBB genotype at 35-40 hpi (Generation 1) in (B) +Choline cultures and (C) in -Choline cultures. (D) Proportion of asexual and gametocyte stages and total parasitemia by HBB genotype at 35-40 hpi (Generation 1) in +Choline cultures. (E) Reticulocytes percentage by HBB genotype. Statistical tests: B, C and E, Beta-binomial GLMs with Bonferroni correction for pairwise comparisons. Significant p -values are shown as: p < 0.05*, p < 0.01**, p < 0.001***; non-significant p -values as: ns. Abbreviations: CTRL, control sample; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; SC, sexual conversion; SCR, sexual conversion rates. Panel A was created in BioRender. Yasmina, D. (2026) https://BioRender.com/ew8vbur .
Techniques Used: In Vitro, Cell Culture, Parasite, Flow Cytometry, Control
![(A) Microscopy-based quantification of parasite stages (rings, trophozoites and schizonts) expressed as percentages of a total of 100 counted parasites per sample. Y-axis: percentage of parasite stages (%); X-axis: HBB genotype groups (HbAA n = 1, HbAC n = 1 and HbAS n = 2, and HbSS n = 2 and HbSC n = 2 donors from a malaria-endemic area in Nanoro, Burkina Faso. Timepoints shown are where progression toward late stages ( i.e., schizont stages) is most clearly observed, namely 35–40 hpi (Generation 0; left graph and Generation 1; right graph). A shift towards earlier parasite stages indicates developmental delay.(B) Developmental progression of individual parasite cultures across timepoints 35–40 hpi (Generation 0), and 25–30 hpi and 35–40 hpi (Generation 1), shown as the proportion of late stages (schizonts) among total infected RBCs (iRBCs) measured by flow cytometry. Data are stratified by HBB genotype: HbAA ( n = 5), HbAC ( n = 5),HbAS ( n = 4), and HbSC ( n = 1) donors from a malaria-endemic area in Nanoro, Burkina Faso; and HbSS ( n = 12), HbSC ( n = 1) and n = 6 control HbAA donors [HbAA CTRL] from a non-endemic area. (C) Representative flow cytometry plots showing the gating strategy for the detection of late parasite stages. Left: total RBCs gated. Right: within the total RBCs gate in left plot, uninfected RBCs (uRBCs) and iRBCs are identified, with iRBCs further gated into early and late parasite stages by Hoechst 33342. Abbreviations: CTRL, control sample; FSC-A, Forward Scatter-Area; G0, Generation 0; G1, Generation 1; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; RBCs, red blood cells; SSC-A, Side Scatter-Area; Timepoint abbreviations are specified in the figure legend.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_3122/pmc13623122/pmc13623122__ppat.1014542.g003.jpg "... among total infected RBCs (iRBCs) measured by flow cytometry. Data are stratified by HBB genotype: HbAA ( ...")
Figure Legend Snippet: (A) Microscopy-based quantification of parasite stages (rings, trophozoites and schizonts) expressed as percentages of a total of 100 counted parasites per sample. Y-axis: percentage of parasite stages (%); X-axis: HBB genotype groups (HbAA n = 1, HbAC n = 1 and HbAS n = 2, and HbSS n = 2 and HbSC n = 2 donors from a malaria-endemic area in Nanoro, Burkina Faso. Timepoints shown are where progression toward late stages ( i.e., schizont stages) is most clearly observed, namely 35–40 hpi (Generation 0; left graph and Generation 1; right graph). A shift towards earlier parasite stages indicates developmental delay.(B) Developmental progression of individual parasite cultures across timepoints 35–40 hpi (Generation 0), and 25–30 hpi and 35–40 hpi (Generation 1), shown as the proportion of late stages (schizonts) among total infected RBCs (iRBCs) measured by flow cytometry. Data are stratified by HBB genotype: HbAA ( n = 5), HbAC ( n = 5),HbAS ( n = 4), and HbSC ( n = 1) donors from a malaria-endemic area in Nanoro, Burkina Faso; and HbSS ( n = 12), HbSC ( n = 1) and n = 6 control HbAA donors [HbAA CTRL] from a non-endemic area. (C) Representative flow cytometry plots showing the gating strategy for the detection of late parasite stages. Left: total RBCs gated. Right: within the total RBCs gate in left plot, uninfected RBCs (uRBCs) and iRBCs are identified, with iRBCs further gated into early and late parasite stages by Hoechst 33342. Abbreviations: CTRL, control sample; FSC-A, Forward Scatter-Area; G0, Generation 0; G1, Generation 1; GLMs, Generalized linear models; Hb, hemoglobin; HBB, human hemoglobin beta; Hpi, hours post-invasion; RBCs, red blood cells; SSC-A, Side Scatter-Area; Timepoint abbreviations are specified in the figure legend.
Techniques Used: Microscopy, Parasite, Infection, Flow Cytometry, Control
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